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ym1 antibody  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc ym1 antibody
    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and <t>Ym1</t> in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.
    Ym1 Antibody, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice"

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-97729-2

    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.
    Figure Legend Snippet: MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

    Techniques Used: Activity Assay, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Western Blot, Control, Software

    MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.
    Figure Legend Snippet: MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Techniques Used: Control, Software

    Antibodies used in Immunofluorescence staining.
    Figure Legend Snippet: Antibodies used in Immunofluorescence staining.

    Techniques Used: Immunofluorescence, Staining, Concentration Assay

    Antibodies used in Western blot.
    Figure Legend Snippet: Antibodies used in Western blot.

    Techniques Used: Western Blot, Concentration Assay



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    Image Search Results


    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 (CHI3L3), Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 (CHI3L3), another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Journal: bioRxiv

    Article Title: Cribriform Plate Microenvironment Assembles a Suppressive Myeloid Network during EAE-induced Neuroinflammation

    doi: 10.64898/2026.01.07.698165

    Figure Lengend Snippet: (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 (CHI3L3), Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 (CHI3L3), another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

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    Techniques: Expressing, Activation Assay, Immunofluorescence, Imaging, Marker, Binding Assay

    UMAP projection (top left) shows major post contact cell types isolated, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Arg1 , Ly6c2 , Ccr2 , Chil3 (CHI3L3), and Pdpn , identifying infiltrating monocyte-derived, alternatively activated macrophages, distinct from T cells ( Cd3e , Ifng ), DCs ( CCR7 , Cd80 ), microglia ( Tmem119 , P2ry12 ), neutrophils ( Ly6g , Cxcr2 ), and proliferating cells ( Mki67 , Top2a ). Gene ontology enrichment analysis (bottom) demonstrates that these post contact macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Journal: bioRxiv

    Article Title: Cribriform Plate Microenvironment Assembles a Suppressive Myeloid Network during EAE-induced Neuroinflammation

    doi: 10.64898/2026.01.07.698165

    Figure Lengend Snippet: UMAP projection (top left) shows major post contact cell types isolated, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Arg1 , Ly6c2 , Ccr2 , Chil3 (CHI3L3), and Pdpn , identifying infiltrating monocyte-derived, alternatively activated macrophages, distinct from T cells ( Cd3e , Ifng ), DCs ( CCR7 , Cd80 ), microglia ( Tmem119 , P2ry12 ), neutrophils ( Ly6g , Cxcr2 ), and proliferating cells ( Mki67 , Top2a ). Gene ontology enrichment analysis (bottom) demonstrates that these post contact macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Article Snippet: The following antibodies were used for immunohistochemistry: Podoplanin PE (eBioscience, Catalog #: 12-5381-80), CD31 Alexa647 (BD Biosciences, Catalog #: 563608), Lyve-1 eFluor660 (Thermo Fisher Scientific, Catalog #: 50-0443-80), MHC II eFluor450 (eBioscience, Catalog #: 48-5321-80), CD11c Alexa488 (Thermo Fisher Scientific, Catalog #: 53-0114-80), YM1 / CHI3L3 (R&D Systems Catalog #: AF2446), Arginase-1 (Invitrogen, Catalog #: PA5-29645), Cleaved Caspase-3 (Abcam, Catalog #: E83-77) .

    Techniques: Isolation, Expressing, Derivative Assay

    MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: MLKL in myofibroblasts affects the activity of macrophages. ( A ) The secretion of IL-6, NO and TNF-α in MLKL +/+ M1ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( B ) The secretion of IL-10, arginase and Ym1 in MLKL +/+ M2ø treated with MLKL +/+ MFbCM, or MLKL −/− MFbCM. ( C )The skin wound tissues of MLKL +/+ and MLKL −/− mice were subjected to immunofluorescence staining for arginase (red), Ym1 (green) and nuclear (DAPI, blue) on the 5, 7 and 10th days after wound injury. Merge represents the composite picture of target protein and nuclear. The immunofluorescence staining was imaged by fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean value of fluorescence intensity was used to perform calculation (a. u., arbitrary unit). ( D ) The protein expression of arginase and Ym1 in wound tissues of MLKL +/+ and MLKL −/− mice were determined by western blot. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD of multiple independent experiments and analyzed by Student t test or one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale label = 20 μm.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Activity Assay, Immunofluorescence, Staining, Fluorescence, Microscopy, Expressing, Western Blot, Control, Software

    MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: MLKL in myofibroblasts affects macrophages activities through PGE 2 . ( A ) The production of IL-6, NO and TNF-α in MLKL +/+ M1 macrophages after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. ( B ) The production of IL-10, arginase and Ym1 in MLKL +/+ M2ø after MLKL −/− MFbCM treatment or MLKL −/− MFbCM plus PGE 2 treatment. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean ± SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test ( n = 4–6). * P < 0.05, ** P < 0.01, *** P < 0.001. ns not significant.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Control, Software

    Antibodies used in Immunofluorescence staining.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: Antibodies used in Immunofluorescence staining.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Immunofluorescence, Staining, Concentration Assay

    Antibodies used in Western blot.

    Journal: Scientific Reports

    Article Title: MLKL is involved in the regulation of skin wound healing and interplay between macrophages and myofibroblasts in mice

    doi: 10.1038/s41598-025-97729-2

    Figure Lengend Snippet: Antibodies used in Western blot.

    Article Snippet: Ym1 , Rabbit polyclonal , 45 kDa , 1:1000 , Stemcell technologies , 60,130.

    Techniques: Western Blot, Concentration Assay

    Antibodies used in immunofluorescence staining

    Journal: Cell Communication and Signaling : CCS

    Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

    doi: 10.1186/s12964-025-02063-9

    Figure Lengend Snippet: Antibodies used in immunofluorescence staining

    Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

    Techniques: Immunofluorescence

    Antibodies used in Western blot

    Journal: Cell Communication and Signaling : CCS

    Article Title: NLRP3: a key regulator of skin wound healing and macrophage-fibroblast interactions in mice

    doi: 10.1186/s12964-025-02063-9

    Figure Lengend Snippet: Antibodies used in Western blot

    Article Snippet: Ym1 , Rabbit polyclonal , , 1:50 , Stemcell technologies , 60,130.

    Techniques: Western Blot, Concentration Assay